DNA cloning tips2


※上記の広告は60日以上更新のないWIKIに表示されています。更新することで広告が下部へ移動します。

In order to do one point mutation
1. look for the restriction enzyme around the place you want to insert.
2.check that is unique.
3.



Quick change mutagenesis
manual



the appropriate amount of vector for retransformation
I am gonna add 50 ng.

trouble shooting





Vector and primers