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There are three ways express DNA in vivo.
1. uteroelectrophoration
2. transgenic mice
3. AAV (adeno-associated virus)



1.PI(Propidium iodide)  staining  neuronal death marker
    PI binds to DNA.
2.NeuN                  staining of nuclear of neurons
   (NeuN is the proteins which is localized in the nuclear in neurons)
3.GFAP                  glia, especially astrocyte, maker
4.Iba1                  microglia marker

immunocytochemistry of hippocampal slice is 10-40 microm
vibratome is difficult to cut less than 40 microm.


AMPAR EPSC(pA) voltage clamp -60 mV
NMDAR EPSC(pA) voltage clamp 40 mV

cLTP reagent.
1. 0mM Mg++
2. 4mM Ca++
3. 50 microM forskolin (cAMP producer)
4. 100 microM picrotoxin (GABAR inhibitor)
5. 100 microM rolipram (PDE-4 inhibitor: keep cAMP active)
うえだ



There are three ways express DNA in vivo.
1. uteroelectrophoration
2. transgenic mice
3. AAV (adeno-associated virus)



1.PI(Propidium iodide)  staining  neuronal death marker
    PI binds to DNA.
2.NeuN                  staining of nuclear of neurons
   (NeuN is the proteins which is localized in the nuclear in neurons)
3.GFAP                  glia, especially astrocyte, maker
4.Iba1                  microglia marker

immunocytochemistry of hippocampal slice is 10-40 microm
vibratome is difficult to cut less than 40 microm.


AMPAR EPSC(pA) voltage clamp -60 mV
NMDAR EPSC(pA) voltage clamp 40 mV